- Emily Fitzroy - Miss Cornelia Van Gorder
- Jack Pickford - Brooks Baliey
- Jewel Carmen - Miss Dale Ogden
- Robert McKim - Dr. H.E. Wells
- Arthur Housman - Richard Fleming
- Louise Fazenda - Lizzie Allen
- Tullio Carminati - Detective Moletti
- Eddie Gribbon - Detective Anderson
- George Beranger - Gideon Bell
- Charles Herzinger - Courtlegh Fleming
- Sojin Kamiyama - Billy the Bulter
- Lee Shumway - as the Unknown
Director Roland West made his cast work at night. "Given the quiet of the studio at midnight, when no other companies are working, and plenty of spooky music from an orchestra, the players really are in a mood to simulate dark deeds".
- Director Roland West remade his film four years later as The Bat Whispers (1930), with Chester Morris and Una Merkel, and also released by United Artists.
- A 1959 remake, The Bat, starring Vincent Price and Agnes Moorehead, was released by Allied Artists.